High throughput sorting is an essential and critical tool in biomedical diagnosis and analysis. We follow two approaches: In hydrodynamic flow fields cells are separated in bulk by viscous lift forces depending on size and mechanical deformability. A complementary technique is based on single cell interrogation. We probe individual fluorescently labelled cells and sort them acoustically at ultra-high throughput (10th of kHz) in multiple channels.
Fluorescence-activated sorting of cells by acoustic streaming
Acoustic Sorting of droplets into 3 different populations based on their fluorescence signal
Fluorescence-Activated SAW-Actuated Cell and Droplet Sorting
Schmid, L., Weitz, D. A., and Franke, T. (2014) Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter. Lab on a Chip , 14(19), pp. 3710-3718. (doi:10.1039/c4lc00588k)
Franke, T., Braunmüller, S., Schmid, L., Wixforth, A., and Weitz, D. A. (2010) Surface acoustic wave actuated cell sorting (SAWACS). Lab on a Chip , 10(6), pp. 789-794. (doi:10.1039/B915522H)
Franke, T., Hoppe, R. H.W., Linsenmann, C., and Zeleke, K. (2013) Numerical simulation of surface acoustic wave actuated cell sorting. Central European Journal of Mathematics, 11(4), pp. 760-778. (doi:10.2478/s11533-012-0165-9)
Franke, T., Abate, A. R., Weitz, D. A., and Wixforth, A. (2009) Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices. Lab on a Chip, 9(18), pp. 2625-2627. (doi:10.1039/B906819H)
Non-Inertial-Lift
Geislinger, T. M., and Franke, T. (2014) Hydrodynamic lift of vesicles and red blood cells in flow — from Fåhræus & Lindqvist to microfluidic cell sorting. Advances in Colloid and Interface Science , 208, pp. 161-176. (doi:10.1016/j.cis.2014.03.002)
Geislinger, T.M., and Franke, T. (2014) Filtern war gestern: Sortierung von zirkulierenden Tumorzellen in Lab-on-a-Chip Systemen. GIT Labor-Fachzeitschrift , 4, pp. 50-52.
Geislinger, T. M., Chan, S., Moll, K., Wixforth, A., Wahlgren, M., and Franke, T. (2014) Label-free microfluidic enrichment of ring-stage Plasmodium falciparum-infected red blood cells using non-inertial hydrodynamic lift. Malaria Journal , 13(1), p. 375. (doi:10.1186/1475-2875-13-375)
Geislinger, T. M., and Franke, T. (2013) Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift. Biomicrofluidics , 7(4), 044120. (doi:10.1063/1.4818907)
Geislinger, T.M., Eggart, B., Braunmüller, S., Schmid, L., and Franke, T. (2012) Separation of blood cells using hydrodynamic lift. Applied Physics Letters , 100(18), p. 183701. (doi:10.1063/1.4709614)
Others
Franke, T., Hoppe, R. H.W., Linsenmann, C., and Zeleke, K. (2013) Numerical simulation of surface acoustic wave actuated cell sorting. Central European Journal of Mathematics, 11(4), pp. 760-778. (doi:10.2478/s11533-012-0165-9)
Franke, T., Abate, A. R., Weitz, D. A., and Wixforth, A. (2009) Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices. Lab on a Chip, 9(18), pp. 2625-2627. (doi:10.1039/B906819H)
