{"id":68,"date":"2015-10-16T00:04:47","date_gmt":"2015-10-16T00:04:47","guid":{"rendered":"http:\/\/frankelab.de\/?p=68"},"modified":"2015-10-16T14:00:23","modified_gmt":"2015-10-16T14:00:23","slug":"sorting","status":"publish","type":"post","link":"http:\/\/frankelab.de\/?p=68","title":{"rendered":"Sorting"},"content":{"rendered":"

High throughput sorting is an essential and critical tool in biomedical diagnosis and analysis. We follow two approaches: In hydrodynamic flow fields cells are separated in bulk by viscous lift forces depending on size and mechanical deformability. A complementary technique is based on single cell interrogation. We probe individual fluorescently labelled cells and sort them acoustically at ultra-high throughput (10th<\/sup> of kHz) in multiple channels.<\/p>\n

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\"Fluorescence-activated
Fluorescence-activated sorting of cells by acoustic streaming<\/figcaption><\/figure>\n

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\"Acoustic
Acoustic Sorting of droplets into 3 different populations based on their fluorescence signal<\/figcaption><\/figure>\n

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Fluorescence-Activated SAW-Actuated Cell and Droplet Sorting<\/h3>\n

Schmid, L., Weitz, D. A., and\u00a0Franke, T.\u00a0(2014)\u00a0Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter.\u00a0Lab on a Chip<\/i>, 14(19), pp. 3710-3718. (doi:10.1039\/c4lc00588k)<\/a><\/p>\n

Franke, T., Braunm\u00fcller, S., Schmid, L., Wixforth, A., and Weitz, D. A. (2010)\u00a0Surface acoustic wave actuated cell sorting (SAWACS).\u00a0Lab on a Chip<\/i>, 10(6), pp. 789-794. (doi:10.1039\/B915522H)<\/a><\/p>\n

Franke, T., Hoppe, R. H.W., Linsenmann, C., and Zeleke, K. (2013) Numerical simulation of surface acoustic wave actuated cell sorting. Central European Journal of Mathematics, 11(4), pp. 760-778. (doi:10.2478\/s11533-012-0165-9) <\/a><\/p>\n

Franke, T., Abate, A. R., Weitz, D. A., and Wixforth, A. (2009) Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices. Lab on a Chip, 9(18), pp. 2625-2627. (doi:10.1039\/B906819H) <\/a><\/p>\n

 <\/p>\n

Non-Inertial-Lift<\/h3>\n

Geislinger, T. M., and\u00a0Franke, T.\u00a0(2014)\u00a0Hydrodynamic lift of vesicles and red blood cells in flow \u2014 from F\u00e5hr\u00e6us & Lindqvist to microfluidic cell sorting.\u00a0Advances in Colloid and Interface Science<\/i>, 208, pp. 161-176. (doi:10.1016\/j.cis.2014.03.002)<\/a><\/p>\n

Geislinger, T.M., and\u00a0Franke, T.\u00a0(2014)\u00a0Filtern war gestern: Sortierung von zirkulierenden Tumorzellen in Lab-on-a-Chip Systemen.\u00a0GIT Labor-Fachzeitschrift<\/i>, 4, pp. 50-52.<\/a><\/p>\n

Geislinger, T. M., Chan, S., Moll, K., Wixforth, A., Wahlgren, M., and\u00a0Franke, T.\u00a0(2014)\u00a0Label-free microfluidic enrichment of ring-stage Plasmodium falciparum-infected red blood cells using non-inertial hydrodynamic lift.\u00a0Malaria Journal<\/i>, 13(1), p. 375. (doi:10.1186\/1475-2875-13-375)<\/a><\/p>\n

Geislinger, T. M., and\u00a0Franke, T.\u00a0(2013)\u00a0Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift.\u00a0Biomicrofluidics<\/i>, 7(4), 044120. (doi:10.1063\/1.4818907)<\/a><\/p>\n

Geislinger, T.M., Eggart, B., Braunm\u00fcller, S.,\u00a0Schmid, L., and\u00a0Franke, T.\u00a0(2012)\u00a0Separation of blood cells using hydrodynamic lift.\u00a0Applied Physics Letters<\/i>, 100(18), p. 183701. (doi:10.1063\/1.4709614)<\/a><\/p>\n

 <\/p>\n

Others<\/h3>\n

Franke, T., Hoppe, R. H.W., Linsenmann, C., and Zeleke, K. (2013) Numerical simulation of surface acoustic wave actuated cell sorting. Central European Journal of Mathematics, 11(4), pp. 760-778. (doi:10.2478\/s11533-012-0165-9)<\/a><\/p>\n

Franke, T., Abate, A. R., Weitz, D. A., and Wixforth, A. (2009) Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices. Lab on a Chip, 9(18), pp. 2625-2627. (doi:10.1039\/B906819H)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

High throughput sorting is an essential and critical tool in biomedical diagnosis and analysis. We follow two approaches: In hydrodynamic flow fields cells are separated in bulk by viscous lift forces depending on size and mechanical deformability. A complementary technique is based on single cell interrogation. We probe individual fluorescently labelled cells and sort them […]<\/p>\n","protected":false},"author":1,"featured_media":266,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-68","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-featured"],"_links":{"self":[{"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/posts\/68","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/frankelab.de\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=68"}],"version-history":[{"count":29,"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/posts\/68\/revisions"}],"predecessor-version":[{"id":383,"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/posts\/68\/revisions\/383"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/frankelab.de\/index.php?rest_route=\/wp\/v2\/media\/266"}],"wp:attachment":[{"href":"http:\/\/frankelab.de\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=68"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/frankelab.de\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=68"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/frankelab.de\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=68"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}